%0 Journal Article
%A GONG Yi-Qin
%A LI Pei
%A LIU Li-Wang*
%A WANG Ming-Xia
%A ZHAO Li-Ping
%T Optimization of RAMP-PCR reaction system in genomic DNA of radish (Raphanus sativus)
%D 2006
%R 10.7525/j.issn.1673-5102.2006.01.022
%J Bulletin of Botanical Research
%P 93-97
%V 26
%N 1
%X Genomic DNA of Radish was analyzed by optimizing the concentration of Mg2+, dNTPs and primer in Random Amplified Microsatellite Polymorphism (RAMP) system. Three different concentration gradients were set respectively, Mg2+ 0.75，1.5，3.0 mmol·L-1；dNTPs 0.05，0.15，0.3 mmol·L-1；primer 0.065，0.2，0.4 μmol·L-1, and the suitable annealing temperature was also screened. An optimization of RAMP reaction system for radish genomic DNA is（20 μL）: dNTPs 0.15 mmol·L-1，Mg2+ 1.5 mmol·L-1，primer 0.2～0.4 μmol·L-1，DNA 10 ng，Taq E 0.8U.The amplification protocol was: 94℃ 3 min，42 cycles for 1 min at 94℃，1 min at 45℃, 1.5 min at 72℃, followed by a final extension of 8 min at 72℃. The suitable system was applied in screening the primer combinations, and the genetic diversity of seven radish varieties was analyzed in RAMP marker system.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2006.01.022