%0 Journal Article
%A CHEN Jing-Zhong
%A GUAN Rui-Ting
%A LIU Ji-Ming
%A TONG Bing-Li
%A WANG Min
%A WU Meng-Yao
%T Genetic Diversity of Critically Endangered Plants <i>Petrocalamus luodianensis</i> from Different Natural Populations
%D 2019
%R 10.7525/j.issn.1673-5102.2019.05.013
%J Bulletin of Botanical Research
%P 740-751
%V 39
%N 5
%X To reveal the endangered reasons of <i>Petrocalamus luodianensis</i>, and provide theoretical support for the protection and utilization of this species, and then form the best protecting strategy for the critically endangered species, we explored the genetic diversity of different natural populations of <i>P.luodianensis</i> and the correlation with environmental factors at the molecular level. The genetic diversity and heritable variation of sixteen natural populations were studied by POPGENE 32 and the technology marked by random amplified polymorphic DNA(RAPD). The results showed that 105 high reproducible, stable and discernible DNA fragments weight from 200-3 000 bp were generated from 8 RAPD primers and 98 polymorphic locis are included, and the <i>PPL</i> is 93.33%. At the species level, the effective number of alleles(<i>N</i><sub>e</sub>) is 1.494 2, the Nei's gene diversity(<i>H</i>) is 0.300 5, and the Shannon's information index(<i>I</i>) is 0.458 6. The Luowan(ZY1) population possessed the highest level of genetic diversity(<i>PPL</i>=60.95%, <i>H</i>=0.232 9 and <i>I</i>=0.345 1), while the Taopo(PT1) population showed the lowest level of genetic diversity(<i>PPL</i>=44.76%, <i>H</i>=0.170 0 and <i>I</i>=0.252 3). In the 16 natural populations, the coefficient of genetic differentiation(<i>G</i><sub>st</sub>) is 0.323 1, the gene flows(<i>N</i><sub>m</sub>) is 1.0478 and the(<i>H</i><sub>SP</sub>-<i>H</i><sub>POP</sub>)/<i>H</i><sub>SP</sub> based on Shannon's Information Index(<i>I</i>) is 0.342 9, a little higher than <i>G</i><sub>st</sub>. Therefore, there is a significant variation and abundant diversity of the <i>P.luodianensis</i>, the intra-population variation is greater than the inter-population, and the genetic variation exists mainly within the intra-population.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2019.05.013